144 research outputs found

    Conclusions of nonoperative management in blunt splenic injuries

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    USMF “N. Testemiţanu”, Clinica Chirurgie Nr.1 “N. Anestiadi”, Clinica ATI „V. Ghereg”, Chişinău, Republica Moldova, Al XI-lea Congres al Asociației Chirurgilor „Nicolae Anestiadi” din Republica Moldova și cea de-a XXXIII-a Reuniune a Chirurgilor din Moldova „Iacomi-Răzeșu” 27-30 septembrie 2011Material şi metode: Studiul prospectiv(2008-2011) – 60 traumatizați cu Leziuni Lienale(LL ) închise; B:F/2:1, vârsta medie 39.97±20,35ani; RTS=7,66±0,5; ISS=19,06±8,95. S-a efectuat: USG(100%) de primă intenție în diagnosticarea hemoperitoneului, TC(80%), laparoscopia(20,27%). În 21(35%) cazuri LL a fost izolată, în 39(65%) – asociată. Rezultate: 13(21,66%) pacienți cu PAs≤80mmHg – 11 politraumatizați cu LL: gr.II(7) și III(5) şi fracturi pelviene, 2 pacienți cu LL izolată s-au stabilizat prin repleție volemică fără suport vasotensiv. Trei politraumatizați cu Glasgow ≤12p. şi LL gr.II(2), gr.III(1), (în 2 cazuri instabili hemodinamic) au fost soluționați nonoperator. Severitatea LL(AAST): gr.I-9(15%), gr.II-24(40%), gr.III-25(41,67%), gr.IV-2(3,33%), cele severe constituind 45%. Hemoperitoneul în LL gr.III a constituit 581,25±158,88ml (400-1000ml), în gr.II – 67,65±113,11ml (p<0,001). Volumul hemotransfuziei în LL gr.III asociate cu locomotor şi/sau torace a fost semnificativ mai mare vs pacienții cu gr.III izolat (648,67±49ml vs 283,33±51ml; p<0,001), iar în gr.II asociat valoarea hemotransfuziei a fost 452,5±379ml în raport cu 0 transfuzii în cele izolate (p<0,05). Eşec s-a înregistrat în 6(10%) cazuri: în 5(8,3%) din cauza LL cu hemoperitoneu 1400±200ml (gr.III(2) cu ruptură în doi timpi, gr.IV(2) izolată cu hemoragie prelungită, gr.II(1) cu pseudoanevrism lienal) şi una(1,66%) din cauza perforației jejunului, splina fiind păstrată. Concluzii: LL de gr.I-III izolate, cât şi asociate pot fi rezolvate cu succes, cele de gr.IV necesită o monitorizare complexă riguroasă. Scăderea hematocritului şi hemotransfuziile prelungite la politraumatizați nu pot prezice nereuşita MNO. Volumul şi extinderea hemoperitoneului poate fi un criteriu orientativ de prognostic al eşecului MNO, însă la volume mai mari de 500ml. Lipsa conştiinței nu este un criteriu de evitare a MNO, laparoscopia în aceste condiții ar putea concretiza reuşita opțiunii.Material and methods: Prospective study(2008-2011) on 60 patients with blunt Splenic Injuries(SI): M:F/2:1, mean age 39,27±20,35 years, 21(35%)-isolated SI, 39(65%)- associated, RTS=7,66±0,5; ISS=19,06±8,95. Diagnostic tools: USG in 100%, CT in 48(80%), laparoscopy in 16(20,27%); Results: 13(21,66%) patients with SBP≤80mmHg: 11 polytrauma patients–IInd degree 7, IIIrd dgr.–5 (SI with pelvic trauma), 2 patients with isolated SI, all were stabilized by fluid perfusions, without vasoactive support. 3 polytrauma patients: II nd dgr.-2, III rd dgr.-1, with GCS≤12p. were successfully treated nonoperatively. The severity of SI(AAST) revealed: Istdgr.-9(15%), IInddgr.- 24(40%), IIIrddgr.-25(41,67%), IVthdgr.-2(3.33%), IIIrd degree prevailed, severe injuries being determined in 45%. Hemoperitoneum volume in IIIrd dgr. SI was 581,25±158,88(400-1000ml), in IInddgr.- 67,65±113,11ml (p<0,001). The blood transfusion volume in IIIrddgr. associated SI with thorax or musculo-skeletal trauma was significantly higher vs isolated SI (648,67±49ml vs 283,33±51ml, p<0,001), while in IInddgr. associated SI the average volume of blood transfusion was 452,5±379ml in contrast to 0 transfusions in isolated SI(p<0,05). NOM failure reported in 6(10%) cases: in 5(8,3%) because of SI with hemoperitoneum 1400±200ml (IIIrddgr. (2)-delayed splenic rupture, IVthdgr.(2)-isolated SI with prolonged bleeding, IInddgr.(1)-lienal pseudoaneurism) and 1(1,66%) failure case caused by jejunal rupture, the spleen being preserved.Conclusions: Both isolated and associated Ist-IIIrddgr. SI can be treated nonoperatively, IVthdgr. SI requiring close and complex monitoring. Reduction of haematocrit and expanded blood transfusions in polytrauma patients could not predict NOM failure. The volume and extent of hemoperitoneum could serve as a failure index of NOM when it exceeds 500ml. Lack of awareness is not a criterion to avoid NOM, laparoscopy reflecting the success of the option

    The Impact of Mixing Modes on Reliability in Longitudinal Studies

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    Mixed-mode designs are increasingly important in surveys, and large longitudinal studies are progressively moving to or considering such a design. In this context, our knowledge regarding the impact of mixing modes on data quality indicators in longitudinal studies is sparse. This study tries to ameliorate this situation by taking advantage of a quasi-experimental design in a longitudinal survey. Using models that estimate reliability for repeated measures, quasi-simplex models, 33 variables are analyzed by comparing a single-mode CAPI design to a sequential CATI-CAPI design. Results show no differences in reliabilities and stabilities across mixed modes either in the wave when the switch was made or in the subsequent waves. Implications and limitations are discussed. </jats:p

    Assessing embryo development using swept source optical coherence tomography

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    A detailed assessment of embryo development would assist biologists with selecting the most suitable embryos for transfer leading to higher pregnancy rates. Currently, only low resolution microscopy is employed to perform this assessment. Although this method delivers some information on the embryo surface morphology, no specific details are shown related to its inner structure. Using a Master-Slave Swept-Source Optical Coherence Tomography (SS-OCT), images of bovine embryos from day 7 after fertilization were collected from different depths. The dynamic changes inside the embryos were examined, in detail and in real-time from several depths. To prove our ability to characterize the morphology, a single embryo was imaged over 26 hours. The embryo was deprived of its life support environment, leading to its death. Over this period, clear morphological changes were observed

    An Integrated TCGA Pan-Cancer Clinical Data Resource to Drive High-Quality Survival Outcome Analytics

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    For a decade, The Cancer Genome Atlas (TCGA) program collected clinicopathologic annotation data along with multi-platform molecular profiles of more than 11,000 human tumors across 33 different cancer types. TCGA clinical data contain key features representing the democratized nature of the data collection process. To ensure proper use of this large clinical dataset associated with genomic features, we developed a standardized dataset named the TCGA Pan-Cancer Clinical Data Resource (TCGA-CDR), which includes four major clinical outcome endpoints. In addition to detailing major challenges and statistical limitations encountered during the effort of integrating the acquired clinical data, we present a summary that includes endpoint usage recommendations for each cancer type. These TCGA-CDR findings appear to be consistent with cancer genomics studies independent of the TCGA effort and provide opportunities for investigating cancer biology using clinical correlates at an unprecedented scale. Analysis of clinicopathologic annotations for over 11,000 cancer patients in the TCGA program leads to the generation of TCGA Clinical Data Resource, which provides recommendations of clinical outcome endpoint usage for 33 cancer types

    Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

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    This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin

    Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

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    Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

    Spatial Organization and Molecular Correlation of Tumor-Infiltrating Lymphocytes Using Deep Learning on Pathology Images

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    Beyond sample curation and basic pathologic characterization, the digitized H&E-stained images of TCGA samples remain underutilized. To highlight this resource, we present mappings of tumorinfiltrating lymphocytes (TILs) based on H&E images from 13 TCGA tumor types. These TIL maps are derived through computational staining using a convolutional neural network trained to classify patches of images. Affinity propagation revealed local spatial structure in TIL patterns and correlation with overall survival. TIL map structural patterns were grouped using standard histopathological parameters. These patterns are enriched in particular T cell subpopulations derived from molecular measures. TIL densities and spatial structure were differentially enriched among tumor types, immune subtypes, and tumor molecular subtypes, implying that spatial infiltrate state could reflect particular tumor cell aberration states. Obtaining spatial lymphocytic patterns linked to the rich genomic characterization of TCGA samples demonstrates one use for the TCGA image archives with insights into the tumor-immune microenvironment

    Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

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    Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN
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